J Drug Target. The direct Coomb’s test after transfusion. MIgG: mono\clonal anti\human globulin; PcIgG: ploy clonal anti\human globulin; C3d: complement 3d; NS: normal saline. F, The urine collected from each mouse in different group within 4?hours. G, The analysis Monooctyl succinate of free haemoglobin in urine. (H\J), The analysis of CREA, BUN and LDH in transfused mice peripheral blood. The model of hemolysis Monooctyl succinate was established through injection of heme from murine destroyed RBCs, followed by (K\M). Mice were injected with supernatant of lysated mice peripheral blood Monooctyl succinate (10L/g) via caudal vein, as HEMEs group. The control group was injected with equal amount of PBS. After 4h, the mice urines were collected and analyzed. HEME: the group of heme treatment; CTRL: the control group. K, The analysis of CREA in blood serum. L, The analysis of BUN in blood serum. M, The analysis of LDH in peripheral blood. N, Western blot analysis of TIM\1 and NGAL protein in RTECs from hemolysis model mice with heme injection. Each data represents 5 mice per group are shown as meanSD. *P?Rabbit Polyclonal to p70 S6 Kinase beta (phospho-Ser423) heme and 25M NAC for 4h to analyze cellular ROS level by flow cytometry. H, Cell viabilities were measured by CCK8 kit, after treated as in (G). Results were representative of five independent experiments. Data are shown as meanSD. *P?
J Drug Target