We found that adult retinal axons have a lower ratio of GM1/GD1a gangliosides on their surface than DRG axons. absence of eCa2+ on Neu3 sialidase activity and axonal regeneration, cultures were incubated with normal growth media devoid of CaCl2, 30 min before axotomy. siRNA transfection Dissociated DRGs were transfected with siRNA 48 h after plating. […]