Day: December 29, 2021

Section were incubated in 4 overnight?C with antibodies against CA9 (1:200, Abcam), Compact disc10 (1:30, Dako), Compact disc44 (1:400, BD biosciences), CXCR4 (1:200, Abcam), MET (1:50, Cell Signaling Technology), VCAM1 (1:250, Abcam), E-Cadherin (1:1000, BD biosciences), Epcam (1:500, Abcam), Ki-67 (1:200, Thermo Fisher), or JAG1 (1:200, Atlas Antibodies) major antibodies and Alexa-conjugated supplementary antibodies (1:200, Dianova)

Section were incubated in 4 overnight?C with antibodies against CA9 (1:200, Abcam), Compact disc10 (1:30, Dako), Compact disc44 (1:400, BD biosciences), CXCR4 (1:200, Abcam), MET (1:50, Cell Signaling Technology), VCAM1 (1:250, Abcam), E-Cadherin (1:1000, BD biosciences), Epcam (1:500, Abcam), Ki-67 (1:200, Thermo Fisher), or JAG1 (1:200, Atlas Antibodies) major antibodies and Alexa-conjugated supplementary antibodies (1:200, […]

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