For the analysis of iPSC gene manifestation in clonal lines RNA was extracted from undifferentiated iPSC cultures and converted into cDNA using the SuperScript Reverse Transcriptase III Kit (Life Technologies) using oligo-dT priming according to the manufacturers instructions. protein. The RP2 individual fibroblasts and iPSC-derived RPE cells showed phenotypic problems in IFT20 localization, Golgi cohesion […]