The expression of vinculin was used as a loading control. and its inhibitor. strain BL21 (DE3) (Stratagene, La Jolla, CA) and subsequently purified using the glutathione-Sepharose 4B batch method (GE Healthcare). translation was performed using the TnT? QuickCoupled transcription/translation systems (Promega, Madison, WI). Plasmid transfections were carried out using FuGENE HD transfection reagent (Roche Applied […]