Cells was grown in 5% CO2/air flow at 37C in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum, 2 mM L-glutamine, 10 mM HEPES, 100 U/ml penicillin G, 100 g/ml streptomycin, and 1 g/ml fungizone. and TNF, which have been implicated PF-5006739 in Th17 reactions, but not IL-12 or IFN.28C32 At present, the mechanisms […]