(C) Rectal temperatures and (D) medical symptoms scores were recorded daily beginning at 0 dpi to 14 dpi

(C) Rectal temperatures and (D) medical symptoms scores were recorded daily beginning at 0 dpi to 14 dpi. GUID:?E32DB3AC-B8AA-419F-8F23-91E18F5E6FB7 Figure 3source data 1: TGEV-specific neutralization antibody in serum. elife-57132-fig3-data1.xlsx (13K) GUID:?D16BD300-073A-4A77-A00A-C5396989690B Number 3source data 2: The percentage of intestinal villus height to the crypt depth in TGEV group. elife-57132-fig3-data2.xlsx (13K) GUID:?F199FC4E-D4DD-4044-B12A-C35F69712E54 Number 4source data 1: PDCoV-specific neutralization antibody in serum. elife-57132-fig4-data1.xlsx (11K) GUID:?FCAB025F-9910-437A-B119-356F215AC9Abdominal Number 4source data 2: The percentage of intestinal villus height to the crypt depth in PDCoV group. elife-57132-fig4-data2.xlsx (13K) GUID:?97A75CDD-0CE0-42E0-B535-3876FDF2F870 Figure 4figure product 1source data 1: The TCID50 detection of PDCoV titer in PAMs. elife-57132-fig4-figsupp1-data1.xlsx (10K) GUID:?B216194D-0B28-442A-8772-73BB95B5ED67 Figure 4figure supplement 1source data 2: The qRT-PCR detection of PDCoV RNA copies in PAMs. elife-57132-fig4-figsupp1-data2.xlsx (10K) GUID:?6869624A-1838-47AB-945E-854865A95EC6 Number 4figure product 1source data 3: WST-8 The western blot detection of the family member PDCoV-N protein level in PAMs. elife-57132-fig4-figsupp1-data3.xlsx (10K) GUID:?AAC36BB8-730D-490C-B607-157A2E25F37E Number 5source data 1: Meat color scores for WT and DKO Ly6a pigs. elife-57132-fig5-data1.xlsx (9.4K) GUID:?95B17BF0-6E1F-4E03-B6E4-B09A8368DE4D Number 5source data 2: Detection of Fe content in longissimus dorsi muscle from WT pigs and DKO pigs. elife-57132-fig5-data2.xlsx (10K) GUID:?98ADBEEB-0F63-4216-9525-A4C795FCD053 Figure 5source data 3: Serum Hp levels of WT and DKO pigs. elife-57132-fig5-data3.xlsx (12K) GUID:?60876816-403F-4A88-890B-DAC7DD11CDF0 Table 1source data 1: Carcass qualities and meat quality characteristics of DKO and WT Large White colored pigs. elife-57132-table1-data1.xlsx (12K) GUID:?DA67F304-996A-45F6-83A4-F09525EA5F79 Table 2source data 1: Total protein, total fat, ash, moisture, and individual mineral content of DKO slim meat and WT slim meat. elife-57132-table2-data1.xlsx (13K) GUID:?A3EA9054-7902-4839-B374-C643CC1008FF Table 3source data 1: Assessment of the concentration, motility, and velocity distribution of the sperm between DKO and WT Large White colored pigs. elife-57132-table3-data1.xlsx (11K) GUID:?411143EB-2EF6-44A2-942D-7EB7352347B5 Source data 1: Amino acid content of DKO lean meat and WT lean meat. elife-57132-data1.xlsx (13K) GUID:?99274984-6396-4C5C-B9A7-64D01D8B91C5 Source data 2: Birth weights and average daily gains of WT pigs and DKO pigs from birth weight to slaughtering weight. elife-57132-data2.xlsx (11K) GUID:?EABBB84D-ED10-42FD-B143-74B8CD44D2C1 Supplementary file 1: Genotypes of cell lines used in somatic cell nuclear transfer. elife-57132-supp1.docx (39K) GUID:?679BEBD6-FD2D-4F48-B72A-38B5B6B59D3D Supplementary file 2: Embryo transfer data for and DKO pigs. elife-57132-supp2.docx (41K) GUID:?162D1157-49EF-43C0-AD87-BCB56B0F4AD5 Supplementary file 3: Potential off-target sites identified for sgRNA and sgRNA. elife-57132-supp3.docx (38K) GUID:?549B38BD-7298-4CD6-AA39-30F16A60543B Supplementary file 4: Amino acid content material of DKO slim meat and WT slim meat. elife-57132-supp4.docx (38K) GUID:?A58953B0-1830-4777-9A7C-4616A4590A96 Supplementary file 5: Primers for genotyping. elife-57132-supp5.docx (35K) GUID:?B85698F8-9EE9-4148-BBBA-EF53F97A0F90 Supplementary file 6: Primers for PCR detection of random integration. elife-57132-supp6.docx (36K) GUID:?D2639D97-4D27-4620-AF4B-0D1DF521F963 Supplementary file 7: Primers for off-target analysis. elife-57132-supp7.docx (39K) GUID:?DB893AEC-9E52-44A1-AAA5-3CD5B83FE2B3 Supplementary file 8: Birth weights and average daily benefits of of WT pigs and DKO pigs WST-8 from birth weight to slaughtering weight. elife-57132-supp8.docx (36K) GUID:?3EEA16BA-9838-47E0-A5E7-B84CEA8F42F6 Transparent reporting form. elife-57132-transrepform.pdf (693K) GUID:?700465C7-BF20-42A4-B7E9-3234F0BC7E91 Data Availability StatementAll data generated or analysed during this study are included in the manuscript and supporting documents. Source data files have been offered for Number 2, Number 3, Number 4, Number 4-figure product 1, Number 5, Table 1, Table 2 and Table 3. Abstract Porcine reproductive and respiratory syndrome disease (PRRSV) and transmissible gastroenteritis disease (TGEV) are two highly infectious and lethal viruses causing major economic deficits to pig production. Here, we statement generation of double-gene-knockout (DKO) pigs harboring edited knockout alleles for known receptor proteins CD163 and pAPN and display that DKO pigs WST-8 are completely resistant to genotype 2 PRRSV and TGEV. We found no variations in meat-production or reproductive-performance qualities between wild-type and DKO pigs, but detected improved iron in DKO muscle mass. Additional infection challenge experiments showed that DKO pigs exhibited decreased susceptibility to porcine deltacoronavirus (PDCoV), therefore offering unprecedented in vivo evidence of pAPN as one of PDCoV receptors. Beyond showing that multiple gene edits can be combined inside a livestock animal to accomplish simultaneous resistance to two major viruses, our research introduces a very important model for looking into infection systems of porcine pathogenic infections that exploit pAPN or Compact disc163 for entrance. knockout pigs. They confirmed a knockout series was totally resistant to genotype 2 PRRSV infections (Whitworth et al., 2016). Subsequently, many laboratories possess generated.

(C) Rectal temperatures and (D) medical symptoms scores were recorded daily beginning at 0 dpi to 14 dpi
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