Successful cloning of the ORFs into pFBq was verified through Sanger sequencing at the Central Analytical DNA Sequencing Facility (Stellenbosch University, South Africa). Open in a separate window Figure 1 Schematic presentation of the baculovirus expression strategies used to generate RV-VLPs.The donor plasmids contains ORFs coding for specific rotavirus proteins (labelled downstream to the promoters […]